Effect of Enzyme Catalese on Hydrogen Peroxide

determination The aim of the Assessment Task 1 is to investigate the effect of 1)temporary workererature, 2)pH and 3)substratum niggardness on the action of enzyme such as catalase on henry bleach. Background knowledge Enzymes are organic catalysts composed of proteins that assist organisms in facilitating metabolic reactions without undergoing whatsoever veer themselves. Enzymes are sensitive to their environment and so must(prenominal) remain within a lasting range of factors ( pH, temperature , subst account concentration etc) for them to function.Any deviations from this static state can result in decreased efficiency or in time the denaturing (destruction) of the enzyme. What hits enzymes 1)Temperature- Enzymes stop working if the temperature nip and tucks in a higher place 40? C. increase the temperature alters the 3D inning and so the enzyme can no longer fit the substrate. 2)pH- They work best in deaf(p) conditions neither acidic nor alkaline. 3)Substrate co ncentration Increasing the substrate concentration, increases the activiy of the enzymes till it reaches an optimal spot beyond which there is no multifariousness in the enzyme acitivity.Catalase Enzyme The activity of an enzyme can be demonstrated development liver, which contains the enzyme, catalase. Hydrogen bleach breaks down slowly to form urine and oxygen. One molecule of Catalase can make love with six million molecules of Hydrogen peroxide in 1 minute. This dislocation happens promptly in the present of the Catalase and Oxygen shove off-key evolves rapidly and can be well-tried with a glowing splint or rising bubbles (variable). Changes in the temperature, acidity (pH) and concentration of the hydrogen peroxide will affect the rate of the reaction.The assert was to have a discharge pipe of just substrate without any enzymes present. The validity would be to examine each variable in isolation without mixing any of the 3 variables namely, the pH, temp and s ubstrate concentration. The amount of catalase and hydrogen peroxide will remain the same in all the test tubes. Hypothesis The system is that since hydrogen peroxide breaks down into water and oxygen gas because of the enzyme, it is expected that with change in temperature of the catalase, oxygen bubbles would form.Apparatus / Equipment used -test tubes & test tube racks -pipettes -Tweezers -Ruler -Water baths (for temperature control) -Ice bucket -Thermometer -Beakers -Hotplates -Measuring cylinder -Vinegar -Bi-Carb soda water -pH paper -pH meters Paper towels to cover up spills -Pen and paper to record results Ingredients used -Liver ( enzyme called catalase) -Hydrogen hydrogen peroxide Equipment setup The test tubes were setup up in a test tube rack. Ice bucket to cool and tropical water bucket to warm were likewise kept in readiness. look into 1 (Temperature) execution 1)I put on the work shirt, goggles, gloves and footgear as a safety measure. 2)I chop up up 3 equal pi eces of liver. 3)I hardened 1 piece of liver into integrity test tube each. 4)I prepared 3 test tubes each containing 10ml of hydrogen peroxide. 5)I setup a water baths with 100 Celcius temperature, for temperature control using the thermometer, to ensure the pay temperature was maintained. 6)I fit(p) 2 test tubes containing liver and hydrogen peroxide each into the water bath. )When the correct temperature was reached, I quickly transferred the liver using tweezers into the test tube containing hydrogen peroxide from the same water bath 8)I looked for any oxygen bubbles rising up in the test tube and measured the rise using a ruler 9)I iterate the above steps with 350 Celcius temperature. 10)I repeated the above steps with 350 Celcius temperature. 11)I allowed the test tube content to cool down before disposing off the liquid waste into the sink with gage of water and the solid waste content carefully into the appropriate bin. 12)I rinsed all setup used and dried them for future use.Results of Experiment 1 It was observed that with 100 C, the temperature was also low and there was not sufficiency heat for Catalase to catalyse the reaction well. At 350C temperature, the bubbles produced froth and it appeared like all the enzymes were catalyzing reactios. When the temperature come up to 500C, the bubbles went down, indicating that the temperature was too high, resulting in a breakdown of the enzyme called denaturation. The results when plotted resulted in a doorbell learnd curve. As temperature increases so to does the energising energy of the enzyme and substrate molecules which randomly collide.The frequency of collisions increases as the temperature increases thus initially change magnitude the rate of reaction. This occurs up to a utmost rate of reaction and the temperature at which the utmost rate of reaction is reached is referred to as the optimum temperature. Beyond the optimum temperature, increasing temperature increases the energisi ng energy of the molecules to the point that the three-dimensional shape of the enzyme can be lost. Thus the shape of its active site changes and can no longer bind to the substrate, reducing the rate of reaction beyond the optimum temperature.